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ELISA |
| Definition: |
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A rapid test where an antibody or antigen is linked to an enzyme as a means of detecting a match between the antibody and antigen.
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| Alternative Names: |
| Enzyme-linked immunoassay; EIA |
| How the test is performed: |
| Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and a tourniquet (an elastic band) or blood pressure cuff is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the tourniquet to distend (fill with blood). A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding. The sample is then taken to the laboratory where it is tested for the target antibody or antigen. If the target substance is present in the sample, the test solution has an intense color reaction generated by the attached enzyme indicating a positive test result. |
| How to prepare for the test: |
| If your child is to have this test performed, it may be helpful to explain how the test will feel, and even practice or demonstrate on a doll. The more familiar your child is with what will happen, and why, the less anxiety he or she will feel. Infants and children: The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experiences, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:
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| How the test will feel: |
| When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing. |
| Why the test is performed: |
| This is a preliminary screening tool used to detect either the presence of antigen or antibody in your blood. It is frequently used to screen for present or past infections. |
| Normal Values: |
| Normal values depend on the type of substance your health care provider is screening for. In some people, a positive result may be normal. |
| What abnormal results mean: |
| Abnormal values depend on the type of substance your health care provider is screening for. In some people, a positive result may be normal. |
| What the risks are: |
| Risks associated with venipuncture are slight: |
| Special considerations: |
| Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others. |
Reviewed By: Michael C. Milone, M.D., Ph.D., Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, PA. Review provided by VeriMed Healthcare Network.
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