Heart, section through the middle
Heart, section through the middle
Pericardial fluid culture
Pericardial fluid culture

Pericardial fluid culture

Definition:
This is a laboratory test to isolate and identify organisms that cause infection from a specimen of pericardial fluid.

Alternative Names:
Culture - pericardial fluid

How the test is performed:

The fluid must first be obtained from the sac surrounding the heart. In some people, a cardiac monitor may be placed prior to the test to monitor for heart disturbances. Patches called electrodes will be placed on the chest similar to an ECG. A chest X-ray or ultrasound may precede the test.

The skin of the chest will be cleansed with antibacterial soap. A trained physician, often a cardiologist, will obtain the sample. A small needle is inserted into the chest between the ribs into the pericardium, the thin sac that surrounds the heart, and a small amount of fluid is withdrawn.

There will be a sensation of pressure and some pain as the needle is inserted into the chest. An ECG and chest X-ray may also follow the procedure. Sometimes the pericardial fluid is obtained during open heart surgery.

Samples of the fluid are placed in various culture media in the laboratory. The media is observed for the growth of colonies of microorganisms (bacteria). Certain biochemical tests can identify certain organisms while others require serological tests or tissue culture to identify organisms.

The availability of results varies from a few hours to several weeks depending on the type of organism.

How to prepare for the test:
Food and fluid will probably be restricted for several hours before the test. A chest X-ray or ultrasound may precede the test to identify the area of fluid collection.

Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experiences, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:
How the test will feel:
There will be some pressure and discomfort associated with the removal of the fluid from the sac surrounding the heart. Your doctor should be able to give you enough pain medicine so that the procedure does not hurt very much.
Why the test is performed:
The test is performed when an infection of the heart is suspected or when a pericardial effusion is present.
Normal Values:
The absence of organisms is normal.
What abnormal results mean:
An infection of the pericardium may be present. The specific organism may be identified. Sensitivity testing can determine effective antimicrobial therapy.

Additional conditions under which the test may be performed:
What the risks are:
There is a risk of puncturing the heart or the lung with this procedure. Introducing infection into the area is another rare, but potential risk.
Special considerations:
Not applicable.

Review Date: 1/7/2002
Reviewed By: Camille Kotton, M.D., Infectious Diseases Division, Massachusetts General Hospital and Brigham and Women's Hospital, Boston, MA. Review provided by VeriMed Healthcare Network.
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