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Herpes viral culture of lesion |
| Definition: |
| This is a test to confirm herpes simplex virus present in a skin lesion. |
| Alternative Names: |
| Culture - herpes simplex virus; Herpes simplex virus culture |
| How the test is performed: |
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A specimen from a skin lesion (often a genital lesion) is collected during the acute stage of symptomatic infection and placed in a special transport media. The specimen may be placed in cell cultures to replicate for identification, or it may be identified by monoclonal antibodies that detect early antigens of herpes simplex virus. Serotyping of the virus (type 1 or 2) may also be done. Results are available within 16 hours to 7 days of receipt of the specimen, depending on the culture method used. |
| How to prepare for the test: |
| The specimen must be collected during an acute infection. Infants and children: The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experiences, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:
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| How the test will feel: |
| The specimen is collected by scraping the suspected skin lesion or aspirating fluid from the lesion. This can cause a scraping or sticking sensation. Sometimes a specimen from the throat or eyes is obtained (usually by rubbing a sterile swab against the tissues). |
| Why the test is performed: |
| The test is performed to confirm herpes simplex infection. The diagnosis is often made by clinical examination rather than diagnostic testing. |
| Normal Values: |
| No growth is normal. |
| What abnormal results mean: |
| Abnormal results may indicate active infection with herpes simplex virus or asymptomatic viral shedding. Herpes genital (genital herpes simplex) is an additional condition under which the test may be performed. |
| What the risks are: |
| The risks are slight bleeding or infection at the skin lesion site. |
| Special considerations: |
| Viral culture for herpes is a poor test and is often falsely negative (but extremely rarely falsely positive). The absence of a positive culture does not confirm that the patient does not have herpes. |
Reviewed By: Camille Kotton, M.D., Infectious Diseases Division, Massachusetts General Hospital and Brigham and Women's Hospital, Boston, MA. Review provided by VeriMed Healthcare Network.
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